EFFECT OF DIFFERENT AMINO ACIDS AT DIFFERENT CON-CENTRATIONS ON MULTIPLICATION AND ROOTING STAGE OF In vitro PROPAGATION OF STRAWBERRIES (Fragaria X Ananassa Duch cv. Chandler)
Abstract
The Present study was carried out for the purpose of enhancing strawberries (Fragaria X Ananassa Duch cv. Chandler) via In vitro regeneration. Since amino acid compounds provide a vital role in plant tissue culturing, this study was conducted for the purpose of identifying the optimum conditions and concentrations from three selected amino acid compounds. Tyrosine, Arginine, and Glutamine were added at different concentrations (0, 25 50 and 100 mg/L), separately and supplemented with standard MS nutrient culture medium. The results demonstrated that tyrosine at (25 mg/L) significantly promoted the shooting and rooting growths as well as the acclimatization stage (in vivo) development. Supplementation of the MS culture medium with arginine and/or glutamine at the assessed concentrations yielded results that were much better than the control culture nutrient medium for the induction of the shooting and rooting stages providing healthy plantlets.References
Ageel, S. and K. Elmeer (2011). Effects of casein hydrolysates and glutamine on callus and somatic embryogenesis of date palm (Phoenix dactylifera L.). New York Science Journal 4: 121-125]. (ISSN: 1554-0200). http://www.sciencepub.net/new york
Amin, A. A., M. Rashad and H. M. H. El-Abagy (2007). Physiological effect Yadav, D. Khanam and F. Raihan (2013). Micropropagation of strawberry (Fragaria X Ananassa) through runner culture. 38: 467-472.
Bayya, M. H. M. (2002). Studies onpropagation of some fruit species by usingtissue culture technique. MSc. Thesis, Faculty of Agriculture, Moshtohor, Zagazig University.
Benson, E. E. (2000). In vitro plant recalcitrance, an introduction. In Vitro Cell. Dev. Biol. Plant, 36: 141-148.
Biswas, M. K., M. Hossain, M. B. Ahmed, U. K. Roy, R. Karim, M. A. Razvy, M. Salahin and R. Islam (2007). Multiple shoots regeneration of strawberry under various colour illuminations. American-Eurasian Journal of Scientific Research, 2: 133-135.
Chukwuemeka, R. E., P. Akomeah and O. Asemota (2005). Somatic embryogenesis in date palm (Phoenix dactylifera L.) from apical meristem tissues from ‘Zebia’ and ‘Loko’ landraces. Afr. J. Biotechnol., 4: 244-246.
Donnoli, R., F. Sunseri, G. Martelli and I. Greco (2001). Somatic embryogenesis, plant regeneration and genetic transformation in Fragaria spp. Acta Hortic., 560: 235-240.
Edwin, F., George M., A. Hall and Geert-Jan De Klerk (2008). The components of plant tissue culture media II: Organic Additions, Osmotic and pH Effects and Support Systems, p. 115-173.
El-Shiaty, O. H., S. F. El-Sharabasy and A. H. Abd El-Kareim (2004). Effect of some amino acids and biotin on callus and proliferation of date palm (Phoenix dactylifera L.) Sewy cultivar. Arab. J. Biotech., 7: 265-272.
Gerdakaneh, M., A Mozafari and A. Siosehmardah (2012). Comparative root colonisation of strawberry cultivars Camarosa and Festival by Fusarium oxysporum f. sp. Fragariae. Plant Soil, 358: 75-89.
Gerdakaneh, M., A. A. Mozafari, A Khalighi and A. Siosehmardah (2009). The effects of carbohydrate source and concentration on somatic embryogenesis of strawberry (Fragaria X Ananassa Duch.). Am-Eurasian J. Agric. Environ. Sci., 6: 76-80.
Han, G. Y., X. F. Wang, G. Y Zhang and Z. Y Ma (2009). Somatic embryogenesis and plant regeneration of recalcitrant cottons (Gossypium hirsutum). Afr. J. Biotechnol., 8: 432 437.
Hasan, M. N. (2010). Micropropagation of strawberry. Int. J. Sutain. Crop Prod., 5: 36-41.
ICAR news, October-December (2005). Indian council of agricultural research, 11, New Delhi, India.
Homhuana, S., B. Kijwijana, P. Wangsomnuka, K. Bodhipadmab and D. W. M. Leungc (2008). Variation of plants derived from indirect somatic embryogenesis in cotyledon explants of papaya. Sci. Asia, 34: 347-352.
John, S. J. and Guha-Mukherjee S. (1997). Plant molecular biology and biotechnology (ed) Tiwari, K. K., Singhal G. S. Narosa, Publishing House, New Delhi, 17-28.
Kadhimi, A., A. Alhasnawi, A. Mohamad, A. Yusoff and C. Zain (2014). Tissue culture and some of the factors affecting them and the micropropagation of strawberry. Life Science Journal, 11: 484-493.
Karlidag, H., E. Yildirim and M. Turan (2009). Salicylic acid ameliorates the adverse effect of salt stress on strawberry. Sci. Agric. Piracicaba. Braz., 66: 180-187.
Kauahal, K., A. K. Nath, P. Kauahal and D. R. Sharam (2004). Studies on somaclonal variation in strawberry (Fragaria × Ananassa Duch.) cultivars. Acta Horticulture, 662: 269-275.
Murashige, T. and F. Skoog (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.
Moradi, K., M. Otroshy and M. R. Azimi (2011). Micropropagation of strawberry by multiple shoots regeneration tissue cultures. Journal of Agricultural Technology, 7: 1755-1763.
Martin-Mex, R., E. Villanueva, T. Herrera and A. Larque-Saaverda (2005). Positive effect of salicylates on the flowering of African violet. Aus. J. Bas. Appl. Sci., 2: 879-883.
Nehra, N. S., K. Kartha, K. Stushnoff and K. L. Giles (1994). Effect of in vitro propagation methods on field performance of two strawberry cultivars. Euphytiea, 76: 107-115.
Oliva, V., A. Castro, I. Herrera, M. Mendiola and F. Miceli (2014). Response surface methodology of glutamine, asparagine and 2,4- Dichlorophenoxyacetic acid for Agave americana L. embryo number and their optimization in a RITA® Automatic Bioreactor System. International Journal of BioPio, A. F. Oliveira and J. D. Ramos (2009). Enraizamento de estacas de oliveirasubmetidas à aplicação de fertilizantes orgânicose AIB. Ciênc Agrotec, 34: 337-344.
Rekh, R., P. Mandave and N. Met (2012). Micropropagation of strawberry cultivar Sweet Charlie through axillary shoots proliferation. Journal of Applied Horticulture, 14(1).
Sarker, R. H., M. M. Barkat, B. Ashapurna, M. Shirin, N. R. Mouful and M. I. Hashem (2003). In vitro regeneration in lentil (Lens exculenta Medik). Plant Tissue Cult., 13: 155-163.
Snedecor, W. and W. Cochran (1980). Statistical methods 7th ed. Iowa State University Press. Ames Iowa.
Yamada, Y., R. Kumpaisal, T. Hashimoto, Y. Sugimoto and Y. Suzuki (1986). Growth and aspartate kinase activity in wheat cell suspension culture: Effects of lysin analogs and aspartate-derived amino acids. Plant Physiol., 27: 607-617.