EXPLORING POLYMORPHISM AND EFFECTS OF THE IGFIR GENE ON PRODUCTIVE LIFE IN BARKI EWES
Abstract
The insulin like growth factor I receptor (IGFIR) gene is known to be involved in the control of the insulin like growth factor I (IGFI) and the insulin like growth factor II (IGFII) through mediating their strong actions that affecting many biological processes (e.g. energy expenditure, metabolism, oxidative stress resistance, cancer suppression, follicle development, ovulation rate,…etc.). These processes proved significant effects on age and reproductive traits of many lived organisms. The allelic and genotypic polymorphisms of the IGFIR gene were identified in 95 Barki ewes using the polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) tool. Also, the effect of variation in IGFIR gene on age and reproductive traits of ewes was measured using three sets of general linear mixed effect models. The obtained results revealed two allelic, A (0.19) and B (0.81) and three genotypes, AA (0.05), AB (0.28) and BB (0.67) polymorphisms. Least square mean analysis revealed a significant statistical effect for the IGFIR genotype on age (P ˂ 0.01), total number of lambs born per ewe (TNLB; P ˂ 0.05) and total number of lambs weaned per ewe (TNLW; P ˂ 0.05). The presence of allele A and absence of allele B was significantly associated with longer age (0.89 year; P ˂ 0.01), higher lambing number (0.70; P ˂ 0.05), increased TNLB (0.86; P ˂ 0.05) and increased TNLW (0.65; P ˂ 0.05). Also, the number of allele A was positively associated with age, TNLB and TNLW. Finding the association of variation in IGFIR gene with age and reproductive traits in Barki sheep may be useful for the prolonged productive life.References
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