ANTI-CLASTOGENIC ACTIVITY OF ROSELLE (Hibiscus sabdariffa) EXTRACT USING A VARIETY OF SHORT-TERM GENOTOXIC BIOASSAYS
Abstract
Nowadays, it has been appeared that there are several advantages for the medical use of hibiscus, which showed the ability to reduce cholesterol level and lipids in animals at laboratory tests in addition to antibiotic oxidation. Thus, the aim of this research is to study its role as anticlastogenic agent upon the chromosomes damage. The calyx and sub-calyx of the Roselle plant has long been recognized as a source of antioxidants. The objective of this study was to investigate the capability of Hibiscus sabdariffa juice to act as anticlastogenic agent by preventing or decreasing chromosomal breaks. In order to achieve such a purpose the genetic material of Mouse (Mus musculus, 2n = 40) and roottip cells of Onion (Allium cepa, 2n = 16) were selected and used employing a variety of shortterm genotoxic bioassays that recommended by EPAUS.The obtained result revealed that Roselle cold extract or syrup treatment had anticlastogenic effect. While hot extract has not. How does this suggested repair system play its role? by activation of cell proliferation, apoptosis; or by interfering with cellular repair system or by all these assumptions. Further research is needed in order to precisely answer this question.References
Abid-Alla, S. A. (2007). Effect of honey bee worker products and some natural phytocompounds on the inhibition of induced mutagenicity and carcinogenicity by some chemical compounds. PhD. Thesis. Fac. Agric. Sab Basha-Alexandria. University, Alexandria.
Adegunloye, B. J., J. O. Omoniyi, O. A. Owolabi, O. P. Ajagbonna, O. A. Sofola and H. A. Coker (1996). Mechanisms of blood pressure lowering effect of the calyx of Hibiscus sabdariffa in rats. Afr. J. Med. Sci., 25: 235-238.
Adler, I. D. (1984). Cytogenetics test in mammals: in mutagenicity testing, apreatical a ppraach (Venitt, S. & Parry, J. M. Eds.) IRL Press, Oxford.
Arimochi, H., T. Kuwahara, U. Vinitketkumuen and Y. Ohnishi (1999). Effect of Roselle (Hibiscus sabdariffa Linn) a Thai medicinal plant, on the mutagenicity of various known mutagens in Salmonella typhimurium and formation of aberrant crypt foci induced by colon carcinogens azoxymethane and 2-amino-1-me-Hyl 6-phenylimidazo (4,5-b) pyridine in F344 rats. Food Chem. Toxicol, 37: 591-601.
Batanouny, K. H., E. Abou Table, M. Shabana and F. Soliman (1999). Wild Medicinal Plants in Egypt. Palm Press, Cairo, pp. 207.
Bolognesi, A. (1997). New ribosome inactivating proteins with polynucleotide: adenosine glycosidase and antiviral activities from Basellarubra L. and Bougainvillea spectabilis Wild. Planta, 203: 422-429.
Brusick, D. (1980). Principles of Genetic Toxicology. Plenum Press; N. Y., pp. 279.
Brusick, D. (1987). Principles of Genetic Toxicology. Plenum Press; N. Y., pp. 284.
Chewonarim, T., T. Kinouchi, K. Kataoka, H. Arimochi, T. Kuwahara, U. Vinitketkumuen and Y Ohnishi (1999). Effect of Roselle (Hibiscus sabdariffa Linn.) a Thai medicinal plant, on the mutagenicity of various known mutagens in Salmonella typhimurium and formation of aberrant crypt foci induced by colon carcinogens azoxymethane and 2-amino-1- me-Hyl 6-phenylimidazo (4,5-b) pyridine in F344 rats. Food and Chemical Toxicology, 37: 591-601.
Chorvatovicova, D. and J. Sandula (1995). Effect of carboxymethyl-chitinglucan on cyclophosphamide induced mutagenicity. Mutat. Res., 346: 43-48.
Dafallah, A. A. and Z. Al-Mustafa (1996). Investigation of the antiinflammatory activity of Acacia nilotica and Hibicus sabdariffa. Am. J. Clin. Med., 24: 263-269.
Duke, J. A. (1985). Handbook of Medicinal Herbs (7th ed.) Livingstone Group Ltd. Edinburgh, 228-229.
Hart, J. W. and H. Engberg-Pedersen (1983). Statistics of the mous bone marrow micronucleus test: counting distribution and evaluation of results. Mutat. Res., 111: 195-207.
Hagmar, L., J. Bjork, A. Sjodin, A. Bergman and E. M. Erfurth (2001). Plasma levels of persistent organohalogens and hormone levels in adult male humans. Arch. Environ. Health, 56: 138-143.
Hirunpanich, V., A. Utaipat, N. P. Molales, N. Bunyapraphtsala, H. Sato, A. Herunsalee and C. Suthisisang (2005). Antioxidant effect of aqueous extracts from dried
calyx of Hibiscus sabdariffa Linn. (Roselle) in vitro using rat lowdensity lipoprotein (LDL). Biol. Pharm. Bull., 28: 481-484.
Morton, J. F. (1987). Roselle: In Fruits of warm climates. Miami, USA, Florida Flair Books, p. 281-286.
Norhaizan, Mohd-Esa, F. S. Hern, A. Ismail and C. L. Yee (2010). Antioxidant activity in different parts of Roselle (Hibiscus sabdariffa L.) extracts and potential exploitation of the seeds. Food Chemistry, 122: 1055-1060.
Onyeneka, P. C., E. O. Ajani, D. A. Ameh and K. S. Gamaniel (1999). Anti hypertensive effect of Roselle (Hibiscus sabdariffa) calyx infusion in spontaneously organohalogens and hormone levels in adult male humans. Arch. Environ. Health, 56: 138-143.
Oud, J. L., J. H. de Jong and D. G. de Rooize (1979). A sequential analysis of meiosis in the male mouse using a restricted spermatocyte population obtained by ahydroxy area/Triaziquone treatment. Chromosoma, 71: 237-248.
Rabello-Gay, M. N. (1991). Teste do micronúcleoemmedulaóssea. In: Mutagênese, Teratogênese e Carcinogênese: Métodos e Critérios de Avaliação, Sociedade Brasileira de Genética (ed), p. 83-90.
Schmid, W. (1975). The micronucleus test. Mutat. Res., 31: 9-15.
Schwartz, H. G. (1974). Preparation of metaphase chromosomes. In: Methods in human cytogenetics (eds: Schwarzacher, H. G. and Wolf, U.) Berlin, Heidelberg, New York. Springer Verlag.
Seehy, M. A. and M. A. Osman (1989). Evaluation of nystatin genotoxicity: human leucocyte culture. Bull. Alex Fac. Med., 25: 1637-1641.
Truswell, A. S. (1992). ABC of Nutrition 2nd (ed).Tavisteek Square Inc. London, 50-93.
Tseng, T. H., E. S. Kao, C. Y. Chou, F. P. Lin, H. W. Wu and C. J. Wang (1997). Protective effect of dried flower extracts of Hibiscus sabdariffa L. against oxidative stress in rat primary hepatocytes. Food Chem. Toxicol., 35: 1159-64.
