MOLECULAR MARKERS ASSOCIATED WITH DROUGHT TOL- ERANCE IN Citrullus colocynthis
Abstract
In the present study Citrullus colocynthis L. (Cucurbitaceae) plants were collected from four different sites (Red sea coast (Elba Mountain), New Valley area, North Sinai, and Saint Katreen) by Egyptian Desert Genebank .Molecular markers associated with drought tolerance were studied by fourteen preselected (ISSR) primers exhibited polymorphism obtained from the DNAs of sixteen samples of C. colocynthis (C1-C4) from the four different sites.
Detection and sequencing of some drought tolerance genes in Citrullus colocynthis using the genomic DNA based on the four plants from different sites. The specific primers of those drought tolerant genes were (Dehydrin gene), (UB gene), (P5CS gene), (PEPKS gene) and (ACT gene), which were succeed to detect some drought tolerant genes among the four locations of Citrullus colocynthis.
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