MUTATIONAL ANALYSIS OF BRAF GENE IN EGYPTIAN HEP ATOCELLULAR CARCINOMA PATIENTS USING NGS

Authors

  • AMAL SAAD ABD EL WAHAAB Department of Molecular Diagnostic and Therapeutic, Genetic Engineering and Biotechnol- ogy Research Institute, University of Sadat City
  • GHADA M. NASR epartment of Molecular Diagnostic and Therapeutic, Genetic Engineering and Biotechnol- ogy Research Institute, University of Sadat City
  • MOHAMED OMAN ABD EL-FATAH Department of Molecular Biology, Genetic Engineering and Biotechnology Research Insti- tute, University of Sadat City
  • MOFEDA ABD EL-SALAM KESHK Department of Molecular Diagnostic and Therapeutic, Genetic Engineering and Biotechnol- ogy Research Institute, University of Sadat City
  • RANDA M. TALAAT Department of Molecular Diagnostic and Therapeutic, Genetic Engineering and Biotechnol- ogy Research Institute, University of Sadat City
  • MUSTAFA A. SAKR Department of Molecular Diagnostic and Therapeutic, Genetic Engineering and Biotechnol- ogy Research Institute, University of Sadat City
  • MOHAMED K. KHALIFA Children Cancer Hospital, 57357
  • EHAB A. AHMED Chemistry Department, Faculty of Science, Cairo University
  • ABDEL RAHMAN A. ABDEL RAHMAN Department of Molecular Diagnostic and Therapeutic, Genetic Engineering and Biotechnol- ogy Research Institute, University of Sadat City
  • OSAMA MEGAHED Department of Molecular Diagnostic and Therapeutic, Genetic Engineering and Biotechnol- ogy Research Institute, University of Sadat City
  • MANAL O. EL HAMSHARY Department of Molecular Diagnostic and Therapeutic, Genetic Engineering and Biotechnol- ogy Research Institute, University of Sadat City

Abstract

Limitations: Found no evidence of portal vein invasion but did associate BRAF mutation to hypertension. Larger tumors tend to have more BRAF mutations. More research, including whole exome sequencing, is required to provide a complete explanation for the genetic changes seen in HCC. The use of NGS led to the discovery of several unique gene mutations in HCC, including both confirmed and disproven mutations. The origin and progression of HCC are best understood because of these findings, which offer new views. Larger patient cohorts are required to fully comprehend BRAF genetic alteration and its impact on the development of HCC.

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Published

2024-07-08

Issue

Vol. 53 No. 1 (2024)

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