MOLECULAR CLONING AND CHARACTERIZATION OF TERPENE SYNTHASE 4 (SgTPS4) GENE FROM Salvia guaranitica PLANT
Abstract
Salvia guaranitica is a medicinal and aromatic plant with highly valued in traditional medicine for its abundance of terpenes, especially the monoterpenes (C10) and sesquiterpenes (C15). Various terpenes were believed to contribute to the many useful biological properties in plants. This study aimed at cloning and functionally characterizes a full length sesquiterpene synthase gene from S. guaranitica. Terpene synthase 4 (SgTPS4) has a complete open reading frame (ORF) of 2289 base pairs encoding a 763 amino acids protein. The phylogenetic tree demonstrates that SgTPS4 protein was clustered into the subfamily TPS-c, which belongs to the angiosperm terpenoid synthase. To examine the function of SgTPS4, we expressed this gene in N. tabacum. Two transgenic lines, designated as OE- SgTPS4 -1 and OE- SgTPS4 -2 were further characterized, both molecularly and functionally. The wild type plants showed a little delayed growth compared to the transgenic plants. Gas chromatography-mass spectrometry analysis of the transgenic plants showed that SgTPS4 was responsible for the production of Bicyclogermacrene. This is the first report of a gene involved in the Bicyclogermacrene as a sesquiterpene from S. guaranitica plant. Ethics approval and consent to participate No investigations were undertaken using humans/human samples in this study. No experimental animals were used to conduct any of the experiments reported in this manuscript. Our study did not involve endangered or protected species.
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