MOLECULAR INSIGHT INTO WADI HAGUL RARE DIVERSITY:Echinops spinosus AND fagonia molis, PLANT SPECIES
Abstract
Genetic studies have been performed to distinguish two plants inhabiting a natural valley renowned as Wadi Hagul of Egypt throwing karyotype and DNA barcode analysis. The two plants were morphologically identified as Echinops spinosus and Fagonia mollis. As a result, the karyotype approach exhibited a significant variant among the certain chromosomal features involving chromosomal number, area of chromosomes, chromosomal length, arm ratio, the position of centromeres, and centromeric index. The chromosomal number of both plants was 2n=18, while the DNA barcode identified their species as E. spinosus of the family Asteraceae and F. mollis of the family Zygophyllaceae based on rbcL phylogenetic analysis. Results indicated that, F. mollis evolutionary tree was rooted into two clads, belonging to family Zygophyllaceae and supported to Fagonia latistipulata (bootstrap support of 93%). Indeed, analysis of rbcL sequence will permit to evaluate the taxonomy and systematic of different plants recovered with a good performance in clarifying genetic diversity within and between populations in the studied plants. In particular, Echinops spinosus and Fagonia mollis showed a minor divergent from the common species, therefore an urgent survey for these genotypes and their conservation is required.
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