GENETIC DIVERSITY OF SELECTED FLAX GENOTYPES (Linu- musitatissimum L.) BASED ON SCOT, ISSR AND RAMP MARKERS
Abstract
Thirteen Start Codon Targeted (SCoT), thirteen inter simple sequence repeat (ISSR) primers and eleven random amplified microsatellite polymorphism (RAMP) primer combinations were used to assess the genetic diversity among twelve Flax (Linum usitatissimum L.) genotypes that are currently active in flax breeding programs in Egypt. They successfully produced a total of 209, 177 and 143 reproducible bands, with a polymorphism percentage of 80.38%, 64.40% and 60.83%, respectively. The highest polymorphism was obtained by SCoT marker with a polymorphic information content (PIC) value of (0.23) and marker index (MI) of (2.96) compared with ISSR markers (0.20 and 1.74, respectively) and RAMP (0.16 and 1.06, respectively). Also, data showed higher SCoT values of observed alleles (Na) 1.82, the number of effective alleles (Ne) 1.51 and the effective multiplex ratio (EMR) 12.9 compared with ISSRs (1.65, 1.41 and 8.7) and RAMP (1.63, 1.34 and 6.00, respectively). On the other hand, Shanon index (I) produced by ISSR marker (0.35) showed higher variability than SCoT (0.25) and RAMP (0.30). However, dendrograms derived from SCoT, ISSR and RAMP marker data separated the 12 flax genotypes into two main clusters. Thus, SCoT marker can be used as an effective complementary method besides ISSR and RAMP for molecular characterization of flax as well as for the determination of genetic relationships between flax cultivars.
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