ISOLATION AND IDENTIFICATION OF A CELLULOLYTIC FUNGUS FROM ANIMAL MANURE
Abstract
Successful conversion of lignocellulose waste to fermentable sugars should open the door for the production of many desired metabolites. Fungi are the best cellulose decomposers in nature. The present study describes the isolation of cellulose-degrading fungal strain, AGE-1, from cow manure. AGE-1 fungal strain was tested for the cellulases production under various pHs, incubation temperatures, incubation time, and carbon sources. The AGE-1 isolate showed the maximum activity for CMCase of 1.44 U/ml ± 0.02 on day 7 when the fungal isolate was incubated at 30 °C. The maximum activity for FPase was on day 7 and it reaches 0.88 U/ml ± 0.03 when the isolate incubated at 30ºC. The strain was molecularly identified using the 18S rRNA gene and ITS as Aspergillus terreus. The strain was tested for cellulases production using solidstate fermentation and showed maximum expression of FPase and CMCase at 30ºC and pH7. Various agricultural wastes were tested as substrates for cellulase production by solid-state fermentation, and sugarcane bagasse (SCB) was the best sole source of carbon for cellulase production followed by wheat straw. The isolated strain can be used as a starting step for degrading agricultural waste that can be used for the production of biofuel and the production of desired metabolites from cellulose.
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