THE IMPACT OF siRNA FEATURES ON ITS FIDELITY AND EF- FICIENCY IN TARGETING SOLUBLE ACID INVERTASE GENE IN SUGARCANE
Abstract
Bioinformatic tools to design a suitable siRNA are an essential step before conducting any laboratory experiment. The identification of highly active siRNA is the corner stone in any silencing application. Five separate steps were used in these studies to test the siRNA efficacy in-silico to evaluate the validation of the siRNA sequence targeting soluble acid invertase gene in sugarcane. The used programs and websites for designing siRNA molecule to silence SAI gene in sugarcane confirmed their validity in designing an efficient, less laboring and accurate siRNA sequence. The subsequent five steps were essential to avoid any side effect on the overall physiological pattern in the plant.References
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