MOLECULAR CHARACTERIZATION AND RESPONSE OF FIVE SOYBEANS GENOTYPES TO PEG TREATMENT
Abstract
Improving of drought tolerant genotypes becomes a principle demand, especially for economical crops like soybean. Five soybean genotypes were treated with different polyethylene glycol 6000 concentrations (5%, 10% and 15%) to determine the potential tolerance of the genotypes under maximum water-deficient conditions. Among the studied genotypes, D89-89-40 and Line 30 were found to be more adaptive to 15% and 10% PEG treatments, respectively. They showed the highest expression for proline content as well as superoxide dismutase, peroxidase and ascorbate peroxidase under water stress. This enabled that D89-89-40 and Line 30 genotypes kept the decrease in lipid peroxidation and electrolyte leakage, consequently the decrease in relative water content to a basal level in response to drought treatments was observed. Semi quantitative analysis of CAB3, DHN1 and DHN2 genes showed increase in the expression level of DHN2 gene in D89-89-40 genotype but kept steady in Line 30 genotype. The slightest reduction in DHN1 and CAB3 expressions among genotypes was recorded in D89-89-40 and Line 30 genotypes accompanied with the slightest decrease in photosynthetic activity and pigment content. The study suggested that D89-89-40 and Line 30 genotypes as drought-tolerant genotypes and nominated them for wide spreading cultivation. The results also assumed that the expression level of DHN2 gene could be used as a selection marker for drought tolerance screening in soybean genotypes.References
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