TRANSFER OF GLUCANASE GENE TO RESIST LATE BLIGHT DISEASE IN POTATO (Solanum tuberosum L.)

Authors

  • HEBA S. SHEBL Agricultural Genetic Engineering Research Institute, AGERI, ARC, Giza, Egypt
  • E. A. METRY Agricultural Genetic Engineering Research Institute, AGERI, ARC, Giza, Egypt
  • M. A. RASHED Genetics Department, Faculty of Agriculture, Ain Shams University, Shoubra Elkheima, Egypt
  • M. A. ABD EL-HAFIEZ Genetics Department, Faculty of Agriculture, Ain Shams University, Shoubra Elkheima, Egypt
  • I. M. ISMAIL Agricultural Genetic Engineering Research Institute, AGERI, ARC, Giza, Egypt

Abstract

Potato late blight is a pandemic disease caused by the highly virulent (Phytophthora infestans) fungus. The regeneration capacity was done among three tested potato cultivars (Spunta, Diamont and Desiree). Two different types of explants (inter-node and leaf) were cultured for calli induction and plant regeneration .The highest value of leaf explants was for Desiree cultivar (80%), which induced from developed callus in the 2,4-D medium. The best value of maximum shoot regeneration was also for Desiree cultivar in the BA, IAA and GA3 media from leaf which proved to be more effective. Then cloning of glucanase gene in pRI plasmid which carrying kanamycin resistance (nptII) gene was perfomed and followed by transformation in Agrobacterium tumefaciens LBA4404 strain which used for plant transfection. Nucleotide and amino acid sequences of transformed Agrobacterium were analyzed. The putative transgenic plantlets genomes and cDNA of the isolated RNA for glucanase gene.

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Published

2018-01-12

Issue

Vol. 46 No. 2 (2017)

Section

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