EVALUATION OF TRANSGENIC POTATO LINES RESISTANCE TO POTATO TUBER MOTH, Phthorimaea operculella (ZELLER)

Authors

  • A. MOHAMMED Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center, 9 Gamaa street Giza, Egypt
  • E. METRY Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center, 9 Gamaa street Giza, Egypt
  • I. M. ISMAIL Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center, 9 Gamaa street Giza, Egypt
  • H. EL-SHISHTAWY Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center, 9 Gamaa street Giza, Egypt
  • GH. A. GAD EL-KARIM Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center, 9 Gamaa street Giza, Egypt
  • T. NASR EL-DIN Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center, 9 Gamaa street Giza, Egypt

Abstract

The potato tuber moth, Phthorimaea operculella (Zeller), is the major destructive insect of potato. The larvae attack foliage and tubers in the field and in storage as well. Over the past years, there was a tremendous effort to produce transgenic potato cultivar that is resistant to the larval infestation of potato tuber moth. ‘Spunta’ potato cultivar was previously transformed with Bt-cry5 gene under 35S promoter construct. Three transgenic ‘Spunta’ lines, ‘Spunta G2’, ‘Spunta G3’ and ‘Spunta 6a-3’, were selected for their high resistance to PTM attack. After 14 years of storage, these three lines are subjected to in vitro micropropagation, tuberization and reevaluation. Both PCR and RT-PCR confirmed the presence of cry5 gene within ‘Spunta’ genome and its expression within potato plant tissues. Amplified fragment length polymorphism (AFLP) analysis revealed that the percentage of polymorphism among the three lines and non-transgenic ranged from 0% to 10.6%. Two lines ‘Spunta G3’ and ‘Spunta 6a-3’ were selected for field agronomical performances as steps for environmental safety as well as PTM challenge. Both lines are highly resistant to PTM compared to the control, only 3% of the yield shows susceptibility to larval penetration of the tubers.

References

Ahmed, A. A. I., M. Y. Hashem, S. M. Mohamed and S. H. S. Khalil (2013). Protection of potato crop against Phthorimaea operculella (Zeller) infestation using frass extract of two noctuid insect pests under laboratory and storage simulation conditions. Archives of Phytopathology and Plant Protection, 46: 2409-2419.

Ali, M. (1993). Effects of cultural practices on reducing field infestation of potato tuber moth (Phthorimaea operculella) and greening of tubers in the Sudan. J. Agric. Sci. Cambr., 121: 187-192.

Barton, K. A. and M. J. Miller (1993). Production of Bacillus thuringiensis insecticidal proteins, p. 297-315. In S. Kung and R. Wu [eds.], Transgenic plants, vol. 1. Engineering and utilization. Academic, San Diego, CA.

Carmona, E. R., A. D. Arencibia, J. Lopez, J. Simpson, D. Vargas and F. Sala (2005). Analysis of genomic variability in transgenic sugarcane plants produced by Agrobacterium tumefaciens infection. Plant Breeding, 124: 33-38.

Canedo, V., J. Benavides, A. Golmirzaie, F. Cisneros, M. Ghislain and A. Langnaoui (1999). Assessing Bttransformed potatoes for potato tuber moth, Phthorimaea operculella (Zeller), management. CIP Program Report 1997-1989, CIP, Lima.

Chandel, G., K. Datta and S. K. Datta (2010). Detection of genomic changes in transgenic Bt rice populations through genetic fingerprinting using amplified fragment length polymorphism (AFLP). GM Crops, 1: 327-336.

Douches, D. S., A. L. Westedt, K. Zarka, and E. J. Grafius (1998). Transformation of CryV-Bt transgene combined with natural resistance mechanisms for resistance to tuber moth in potato (Solanum tuberosum L.) HortScience, 33: 1053-1056.

Douches, D. S., J. J. Coombs, K. J. Felcher, M. Zuehlke, W. Pett, Z. Szendrei and J. Brink (2013). Segregation and efficacy of the cry1Ia1 gene for control of potato tuberworm in four populations of cultivated potato. J. Econ. Entomol., 106: 1025-1028.

Douches, D. S., J. A. Brink, H. Quemada, W. Pett, M. Koch, D. Visser, K. Maredia and K. Zarka (2008). Commercialization of potato tuber moth resistant potatoes in South Africa. Tropical Agriculture - Advances in Crop Research, 10: 139-147.

Douches, D., W. Pett, D. Visser, F. Santos, J. Coombs, E. Grafius, W. Li, E. A. Metry, T. N. El-Din and M. Madkour (2004). Field and storage testing Bt potatoes for resistance to potato tuberworm (Lepidoptera: Gelichiidae). J. Econ. Entomol., 97: 1425-1431.

Douches, D. S., W. Pett, D. Visser, J. Coombs, K. Zarka, G. Bothma, J. Brink, K. Felcher, M. Koch and H. Quemada (2010). Field and storage evaluations of ‘Spunta G2’ for resistance to potato tuber moth and agronomic performance. J. Amer. Soc. Hort. Sci. 135: 333-340.

Essmate, M., R. Von Arex, P. Ewell, J. Goueder, A. B. Temine and M. Cheikh (1988). Aspects techniques et economiques du problem de la teigne et du stockage des pommes de terre de saison en Tunisia. Ann. Inst. Nt. Res. Agron. Tunis, 61: 1-50.

Goldson, S. L. and R. M. Emberson (1985). The potato moth Phthorimaea operculella (Zeller): Its habits, damage potential and management. Special Publ., Agron. Soc., N. Z., Christchurch, New Zealand, p. 61-66.

Hagh, Z. G., H. Rahnama, J. Panahandeh, B. B. K. Rouz, K. M. A. Jafari and N. Mahna (2009). Green-tissue-specific, C4-PEPC-promoter-driven expression of Cry1Ab makes transgenic potato plants resistant to tuber moth (Phthorimaea operculella, Zeller). Plant Cell. Rep., 28: 1869-1879.

Huiqun, W. (2009). Effects of chlorocholine chloride on phytohormones and photosynthesis characteristics in potato (Solanum tuberosum L). J. Plant Growth Regul., 28: 21-27.

Hussein, Ebtissam H. A., S. S. Adawy, S. E. M. Ismail and H. A. El-Itriby (2005). Molecular characterization of some Egyption date palm germplasm using RAPD and ISSR markers. Arab J. Biotech., 8: 83-98.

Jansens, S., M. Cornlissen, R. DeClercq, A. Reynaerts and M. Peferoen (1995). Phthorimaea operculella (Lepidoptera: Gelechiidae) resistance in potato by expression of the Bacillus thuringiensis CryIA(b) insecticidal protein. J. Econ. Entomol., 88: 1469-1476.

Koziel, M. G., G. L. Beland, C. Bowman, N. B. Carozzi, R. Crenshaw, L. Crossland, J. Dawson, N. Desai, M. Hill, S. Kadwell, K. Launis, K. Lewis, D. Maddox, K. McPherson, M. R. Meghji, E. Merlin, R. Rhodes, G. W. Warren, M. Wright and S. V. Evola (1993). Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. Biotechnology, 11: 194-200.

Metry, E. A., G. M. El-Moghazy, M. S. Masoud, M. H. Youssef, T. A. Omar, T. Yehya, S. A. Mahfouz, A. M. Shokry, M. F. Emara, A. S. Hamza, T. N. El-Din and H. A. El-Itriby (2008). Nutritional and biological evaluation of transgenic potato lines resistant to potato tuber moth. Egypt. J. Genet. Cytol., 37: 141-152.

Meiyalaghan, S., J. M. E. Jacobs, R. C. Butler, S. D. Wratten and A. J. Conner (2006). Expression of cry1Ac9 and cry9Aa2 genes under a potato light-inducible Lhca3 promoter in transgenic potatoes for tuber moth resistance. Euphytica, 147: 297-309.

Mohammed, A. D., S. Douches, W. Pett, E. Grafius, J. Coombs, W. Liswidowati, W. Li and M. A. Madkour (2000). Evaluation of potato tuber moth (Lepidoptera: Gelechiidae) resistance in tubers of Bt-cry5 transgenic potato lines. J. Econ. Entomol., 93: 472-476.

Murashige, T. and F. Skoog (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiological Plantarium, 15: 473-497.

Roca, W. M., N. O. Espinoza, M. R. Roca and J. E. Bryan (1978). A tissue culture method for the rapid propagation of potatoes. American Potato J., 55: 691-710.

Ross, H. (1986). Potato breeding and per-spectives. Verlag PareyPress, Berlin, Germany.

Saxena, A. P. and S. M. A. Rizvi. (1974). Insect pest problems of potatoes in India. J. Indian Potato Assoc., 1: 45-50.

Visser, D., W. Pett and D. Douches (2013). Developing Transgenic BT-Potato in South Africa: Experiences with Field Trial Protocols. Acta Hort., 974: 51-56.

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2016-01-23

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