EVALUATION OF THE GENETIC STABILITY OF In vitro- PROPAGATED Paulownia tomentosa USING DNA-BASED MARKERS
Abstract
The genetic stability of in vitro-raised Paulownia tomentosa clones was assessed by using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSRs) markers. Out of 12 RAPD and seven ISSR primers screened, only eight RAPD and four ISSR primers produced clear, reproducible and scorable bands. The seven RAPD primers produced 47 distinct and scorable bands. The molecular size of the total 22 fragments generated by RAPD technique primers ranged from ≈ 97-2300 bp.
The number of scorable bands for ISSR primers varied from 3 (ISSR-14) to 8 (ISSR-07). The molecular size of PCR products generated 22 fragments by these ISSR ranged from ≈280 to1390 bp. The number of bands generated per primer was greater in ISSR than RAPD. All banding profiles from micropro-pagated plants were monomorphic and similar to those of the mother plant. A similarity matrix based on Jaccard’s coefficient revealed that the pair-wise value between the mother and the in vitro-raised plantlets was 1, indicating 100% similarity. This confirmed the true-to-type nature of the in vitro-raised clones.
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