ISOLATION, IDENTIFICATION AND BIOCONTROL POTENTIAL OF NATIVE SOIL-DERIVED Trichoderma spp.

Authors

  • NEHAL ATTA Genetics Department, Faculty of Agriculture, Menoufia University, Shibin El-Kom
  • ABDELMEGID I FAHMI Genetics Department, Faculty of Agriculture, Menoufia University, Shibin El-Kom
  • KHALID S. ABDEL-LATEIF Genetics Department, Faculty of Agriculture, Menoufia University, Shibin El-Kom
  • HESHAM H. NAGATY Genetics Department, Faculty of Agriculture, Menoufia University, Shibin El-Kom
  • ENAS M. ABD EL-GHANY Genetics Department, Faculty of Agriculture, Menoufia University, Shibin El-Kom

Abstract

This research aimed to specifically identify 31 isolates at the species level using morphological characteristics combined with DNA barcoding sequence analysis of full-fragment rDNA ITS1 and ITS2 gene regions. The identified species were determined to be T. harzianum (23 strains), T. asperellum (6 strains), and T. longibrachiatum (2 strains). The phylogenetic analysis of the 31 isolates of the ITS region revealed that they formed four clades. Three of them included the isolates of each specific species, while a separate clade included two different isolates. The two isolates MNF-NAH-Tricho5 and MNF-NAH-Tricho30 presented the highest antifungal activity against Rhizoctonia solani (71.43 and 64.29%) and Macrophomina phaseolina (84.1 and 85.13%) according to the dual culture assay. Finally, various strategies to combat phytopathogens, including competition, antibiosis, or parasitism, have been developed for Trichoderma isolates. The overall study confirmed that the MNF-NAH- Tricho5 and MNF-NAH-Tricho30 strains may serve as prospective biocontrol agents to combat Rhizoctonia solani and Macrophomina phaseolina in environmental applications.

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Published

2024-11-26

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Vol. 54 No. 1 (2025)

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