The first record of Apis mellifera jemenitica IN EGYPT AS AN EXOTIC RACE
Abstract
The Arabian or Nubian honeybee, scientifically known as Apis mellifera jemenitica (A. m. jemenitica), is a distinct subspecies of the western honeybee. A. m. jemenitica is indigenous to several tropical locations, including Saudi Arabia, Sudan, and Somalia. A. m. jemenitica has not been recorded before in Egypt. Therefore, this work aimed to validate the visual observation of the beginning of the appearance of Yemeni bees in Egypt. Fifteen morphometric measures and two molecular markers (Simple Sequence Repeats; SSRs and 16SrRNA) were used to establish morphological and genetic identity among A. m. jemenitica samples collected in Egypt and those acquired in Saudi Arabia as a reference. Morphometric and molecular analyses revealed close similarities between the studied bees, which confirm our hypothesis about the presence of Yemeni bees in Egypt.
References
Ahmed A., Mohammad A., Yehya A. and Nuru A., (2012). Morphometric diversity of indigenous honeybees, Apis mellifera (linnaeus, 1758). in Saudi Arabia: (Insecta: Apidae). Zool. Middle East 57: 97-103.
Alabdali E. A., Ghramh H. A., Ibrahim E. H., Ahmad Z. and Asiri A. N., (2021). Characterization of the native honey bee (Apis mellifera jemenitica) in the south western region of Saudi Arabia using morphometric and genetic (mtDNA COI) characteristics. Saudi J. Biol. Sci., 28: 2278-2284.
Alattal Y., Alsharhi M., Alghamdi A., Alfaify S., Migdadi H. and Ansari M., (2014). Characterization of the native honey bee subspecies in Saudi Arabia using the mtDNA COI–COII intergenic region and morphometric characteristics. Bull Insectol., 67:31-37.
Alburaki M., Moulin S., Legout H., Alburaki A. and Garnery L., (2011). Mitochondrial structure of Eastern honeybee populations from Syria, Lebanon and Iraq. Apidologie, 42: 628-641.
Alghamdi A. and Alattal Y., (2021). Assessment of genetic variation in Apis mellifera jemenitica (Hymenoptera: Apidae) using Cytochrome Oxidase I gene sequences. Saudi J. Biol Sci., 28 (11): 6586-6591.
Al-Ghamdi A. and Nuru A., (2013). Bee-keeping in the Kingdom of Saudi Arabia: past and present practices. Bee World, 90 (2): 26-29.
Al-Ghamdi A. A., Al-Ghamdi M. S., Ahmed A. M., Mohamed A.S. A., Shaker G. H., Ansari M. J., Dorrah M. A., Khan K. A. and Ayaad T. H., (2021). Immune investigation of the honeybee Apis mellifera jemenitica broods: a step toward production of a bee-derived antibiotic against the American foulbrood. Saudi J. Biol. Sci., 28 (3): 1528-1538.
Al-Ghamdi A. A., Nuru A., Khanbash M. S. and Smith D. R., (2013). Geographical distribution and population variation of Apis mellifera jemenitica Ruttner. J. Apic. Res., 52 (3): 124-133.
Al-Ghamdi A. K., Khan A., Al-Abbadi A. A., Ghramh H. A., Ahmed A. M. and Ansari M. J., (2020). In vitro antagonistic potential of gut bacteria isolated from indigenous honeybee race of Saudi Arabia against Paenibacillus larvae. J. Apic. Res., 59 (5): 825-833.
Alqarni A. S., Hannan M. A., Owayss A. A. and Engel M. S., (2011). The indigenous honey bees of Saudi Arabia (Hymenoptera, Apidae, Apis mellifera jemenitica Ruttner): Their natural history and role in beekeeping. ZooKeys, 134: 83-98. doi: 10.3897/zookeys.134.1677
Alqarni A. S. (2011). Beekeeping in Saudi Arabia: current and future. Saudi Society for Agricultural Sciences Riyadh King Saud Univ., 21, 40 (in Arabic).
Chen C., Liu Z., Pan Q., Chen X., Wang H., Guo H., Liu S., Lu H., Tian S.,
Li R. and Shi W. (2016). Genomic analyses reveal demographic history and temperate adaptation of the newly discovered honey bee sub-species Apis mellifera sinisxinyuan. ssp. Mol. Biol. Evol., 33, 1337- 1348.
Cornuet J. and Garnery L., (1991). Mitochondrial DNA variability in honeybees and its phylogeographic implications. Apidologie, 22: 627-642.
Eid Kh S. A., Marei G. I. Kh. and Abd-Elrasol M. A., (2011). Acute toxicity of some biopesticides and their effects on acetylcholinesterase of honey bee (Apis mellifera) workers. J. Plant Prot. and Path., Mansoura Univ., 2(10): 805-827.
Eimanifar A., Brooks S. A., Bustamante T. and Ellis J. D., (2018). Population genomics and morphometric assignment of western honey bees (Apis mellifera L.) in the Republic of South Africa. BMC Genomics 19: 615-641. https://doi.org/10.1186/s12864-018-4998-x
El-Bermawy S. M., Ahmed, K. S., Al-Gohary H. Z. and Bayomy A. M., (2012). Biochemical and molecular characterization for three subspecies of honey bee worker, Apis mellifera L. (Hymenoptera: Api- dae) in Egypt. Egypt. Acad. J. Biol. Sci. A. Entomol., 5: 103-115.
Garnery L., Franck, P., Baudry E., Vautrin D., Cornuet J. M., and Solignac M. (1998). Genetic diversity of the west European honey bee (Apis mellifera mellifera and A. m. iberica) II. Microsatellite loci. Genetics Selection Evolution, 30:49- 74.
Haddad N., Meixner M., Fuchs S., Migdadi H., Garnery L. and Sheppard W., (2009). Mitochondrial DNA support for genetic reserves of Apis mellifera syriaca in Jordan. Journal of Apicultural Research and Bee World, 48:19-22.
Horth L., Haddad N. and Nasher A., (2017). Apis mellifera jemenitica in Yemen. Bee World 94: 66-68. https://doi.org/10.1080/0005772x.2017.1345225
Khan K. A., Ansari M. J., Al-Ghamdi A., Nuru A., Harakeh S. and Iqbal J., (2017). Investigation of gut microbial communities associated with indigenous honey bee (Apis mellifera jemenitica) from two different eco-regions of Saudi Arabia. Saudi J. Biol. Sci. 24, 1061-1068.
Paplauskiene V., Ceksteryte V., Pasakin-skiene I., Tamasauskiene D. and Racye J., (2006). The use of ISSR method for the assessment of bee genetic diversity. Biologica., 3: 16-20.
Rouhani M., Vahedi H. and Marefat A., (2018). Genetic variation of yellow pistachio hard scale, Lepidosaphes pistaciae Archangelskaya (Hem.: Diaspididae) populations in Ker- man province, Iran revealed by ISSR markers. Cell. Mol. Biol., 64 (11): 20-24.
Ruttner F. (1988). Biogeography and taxonomy of honeybees. Springer- Verlag Berlin Heidelberg GmbH, 288. https://doi.org/10.1016/0169-5347 (89): 90176-6.
Ruttner F., Tassencourt L. and Louveaux J., (1978). Biometrical statistical analysis of the geographic variability of Apis mellifera L. Apidologie 9, 363-382.
Shouhani H., Dousti A. and Radjabi R., (2014). Application of ISSR to study the genetic diversity of honeybee (Apis mellifera L.) populations in some areas of Iran. Journal of Bioscience and Biotechnology, 3(2): 127-131.
Taha, E. K. A. and AL-Kahtani S. N., (2019). Comparison of the activity and productivity of Carniolan (Apis mellifera carnica Pollmann) and Yemeni (Apis mel- lifera jemenitica Ruttner) subspecies under environmental conditions of the AlAh- sa oasis of eastern Saudi Arabia. Saudi J. Biol. Sci., 26: 681-687.