Effect of Heat Shock on Some Genes Involved in Heat Tolerance System in Barely
Abstract
Sever climatic changes, especially high temperature, is one of the most important abiotic factors defining the yield potential of temperate cereal crops such as barley. In this work, 4 barley genotypes were used to study the differential response to heat shock. Physiological data pointed that the sensitive genotype showed high leakage and lower electric conductivity. The sensitive genotype (G129) showed high lipid peroxidation compared with G134, the tolerant one. The quantitative PCR analysis for the studied heat shock proteins and transcription factors showed that the level of gene expression of HSP70 was significantly increased after a short time under HS in the sensitive genotype, while a slight increase was observed in the tolerant genotype. The HSP90 showed up regulation in the sensitive genotype G129 under HS condition, but the tolerant genotype G134 showed quite an increase in comparison with control plants. The regulator HSFA1 showed higher expression level in the tol- erant genotype G134 comparing with G129. The expression level of HSFA2 was higher in the sensitive genotype (G135), while moderate high expression in the tolerant genotype G134.
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