PHYLOGENETIC RELATIONSHIPS OF SOME BARLEY (Hordeum vulgare L.) ACCESSIONS IN EGYPT
Abstract
Phylogenetic relationships of eleven accessions of Hordeum vulgare L. collected from different regions of Egypt were assessed. Fifteen quantitative morphological traits were used, the measured data were evaluated statistically using ANOVA, phylogenetic trees were constructed using UPGMA. Also, simple sequence repeats (SSRs) and start codon targeted (SCoT) molecular marker techniques were used for DNA fingerprinting and assessing genetic diversity and phylogenetic relationships in barley germplasm. The results showed that SSR primers produced 50 bands ranged in size between 50-722 bp with 64% polymorphism percentage and SCoT primers produced 143 bands, ranged in size between 165-2060 bp with 72% polymorphism percentage. Polymorphic information content PIC was 0.44 and 0.82 for SSRs and SCoT, respectively. UPGMA dendrogram was divided into two clusters by each of morphological traits, SSRs, and SCoT analysis. Genetic similarity matrix was examined with Jacard’s coefficient, maximum similarity was found between B7 and B8 (0.98%) with quantitative morphological analysis, between B3 and B4 (94%) with SSRs analysis and between B9 and B11 (80%) with SCoT analysis. However, combined cluster analysis between the three components together revealed better resolution for distinction between these taxa.
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