ANALYSING ASSOCIATION OF TMEM154, CCR5 AND ZNF389 VARIANTS WITH MAEDI-VISNA RESISTANCE IN GERMAN SHEEPS
Abstract
Maedi visna disease considerably spreads among German sheep flocks. Variants in or near the genes TMEM154, CCR5, and ZNF389 have been reported to be associated with the serological status or the provirus concentration of ovine lentivirus (OvLV) in U.S. sheep. The first goal of present study was to assess these markers for association with SRLV susceptibility in German sheep breeds. The second goal was to evaluate associations of those markers with MV antibody titer in MV-positive German sheep flocks. A total of 306 DNA samples were divided in two sets according to the purpose in this study. In the set 1 of less and highly susceptible sheep breeds, mean frequencies of putatively protective alleles were 75.4 and 21.1 % (TMEM154 35K, P < 0.000), 14.9 and 5.8 % (CCR5 deletion, P < 0.05) and 46.3 and 54,7 % (ZNF389 insertion, P > 0.05), respectively. In the set 2 of serologically (ELISA) MV-positive and -negative sheep, mean frequencies of putatively protective alleles were 25.5 and 63.4 % (TMEM154 35K, P < 0.000), 15.2 and 20.2 % (CCR5 deletion, P > 0.05), and 53.6 and 49.1 % (ZNF389 insertion, P > 0.05), respectively. According to these results, TMEM154 (E35K) seems to be a more promising marker for selection against MV susceptibility in the German sheep population. These findings warrant further study.
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