MOLECULAR GENETIC IDENTIFICATION OF SOME CAMEL BREEDS IN EGYPT

Authors

  • ALIA A. EL-SEOUDY Dept. of Genetics. Fac. Agric., Ain Shams Univ., Cairo, Egypt Ain Shams Center for Genetic Engineering and Biotechnology, Ain Shams Univ., Cairo, Egypt
  • A. Z. ABDELSALAM1 Dept. of Genetics. Fac. Agric., Ain Shams Univ., Cairo, Egypt Ain Shams Center for Genetic Engineering and Biotechnology, Ain Shams Univ., Cairo, Egypt
  • E. E. THARWAT Dept. of Animal Production, Fac. Agric., Ain Shams Univ., Cairo, Egypt
  • FATMA A. M. ABDELSALAM Ain Shams Center for Genetic Engineering and Biotechnology, Ain Shams Univ., Cairo, Egypt

Abstract

The main objective of the present study is to characterize the three camel breeds (Sudani, Falahi and Maghrabi), using different genetic criteria. Native protein profiles of plasma showed slight differences between the examined animals. Six isozyme system patterns; Carboxylesterase, Acid phosphates, Malate dehydrogenase, Alcohol dehydrogenase, Glycerol-3-phosphat dehydrogenase and Phosphogluconate dehydrogenase were used. The isozymes analysis revealed that, Falahi females and males showed the highest similarity followed by Maghrabi. While, the lowest similarity was observed for Sudani breed. ISSR-PCR gave different DNA markers using fourteen primers. The presence of different amplified DNA segments in the studied breeds could be used as genetic markers for each breed.

References

Bleile, D., M. Foster, J. Brady and J. Harrison (1975). Identification of essential arginyl residues in Cytoplasmic Malate Dehydrogenase with Butanedione. J. Biol. Chem., 250: 6222.

Bollag, M. and J. Edelstein (1994). Protein Methods. Fourth Edition. Wiley-Liss, Inc., 605 Third Avenue, New York.

FAO (2003). Production Yearbook. Vol. 56, Rome.

Mordacq John C. and Roberta W. Ellington (1994). Polyacrylamide Gel Electrophoresis (PAGE) of Blood Proteins. Association for Biology Laboratory Education (ABLE) http://www.zoo.utoronto.ca/able.

Richardson, B. J., P. M. Rogers and G. M. Hewitt, (1980). Ecological genetics of the Wild Rabbit in Australia. Aust. J. Biol. Sci., 33: 371-83.

Sambrook, J., E. F. Fritsh and T. Maniatis (1989) Molecular cloning, a laboratory manual. Cold spring harbor laboratory press, New York.

Wardeh, M. F., A. A. Zaied, H. S. Horier and R. T. Wilson (1990). Camel breeds in Arab Africa. Proc. Of the International Conference on Camel Production and Improvement, 10- 13 December, 1990, Tobruk, Libya, 78-86.

Williams, L. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski and S. V. Tingey (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nuc. Aci. Res., 18: 6531- 6535.

Downloads

Published

2016-01-13

Issue

Vol. 37 No. 2 (2008)

Section

Articles

Most read articles by the same author(s)