CHARACTERIZATION OF TWO Β-DEFENSINS (BNBD5 AND BNBD10) IN EGYPTIAN NATIVE AND FRISIAN CROSSBRED CATTLE
Abstract
Two β-Defensin antimicrobial peptides namely bovine neutrophil β- defensin 5 and 10 (BNBD5 and BNBD10) were investigated in Native and Frisian crossbred cattle reared in Egypt. The two antimicrobial peptides were tested for reaction with cDNA of various cattle tissues such as lung, trachea, liver, intestine, blood, muscle, testis and lymph node using PCR. BNBD5 was positive in lung, trachea and liver of Frisian crossbred but only in lung and trachea of native cattle. BNBD10 was positive in lung and trachea of Frisian crossbred, whereas in native cattle it was expressed in lung, trachea, liver and intestine.
CLUSTAL W (1.83) multiple nucleotide sequence alignment between BNBD5 amplicons of Frisian crossbred, native cattle and of Bos taurus (AF014108) showed 64% alignment between Frisian crossbred and native, 77% between Frisian crossbred and Bos taurus and 67% between native and Bos taurus. CLUSTAL analysis for BNBD10 sequences showed 93% alignment between Frisian crossbred and native, 93% between Frisian crossbred and Bos taurus Exon 1 and 2 (AJ56799), and 90% between native and Bos taurus (AJ56799).
Some point mutations were observed in BNBD5 rather than BNBD10 in native and Frisian crossbred cattle. BNBD5 alignments showed three deletions and two stop codons in Frisian crossbred cattle, whereas in native cattle three deletions and four stop codons were detected. No stop codons were detected in BNBD10; only two insertions were detected in crossbred and native cattle in addition to one deletion in the latter. The above findings were reflected on the amino acid translated sequences, where lower homologies and higher tendency of BNBD5 to mutations than BNBD10 were detected
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