EVALUATION AND GENETIC DIVERSITY OF ELEVEN SESAME LINES
Abstract
This investigation was carried out to identify eleven sesame lines during two successive seasons, 2011 and 2012. Results revealed that line 117-7 surpassed the other genotypes in plant height, length of fruiting zone, number of capsules/pl., seed yield/pl., oil yield /fed., accelerated ageing germination and crude protein, while, line 112-5 gave the maximum estimates for number of branches/pl. and crude oil. Line 111-6 recorded high seed yield/fed. and seedling dry weight. Line 117-1 has the highest germination percentage, seed vigor index, shoot length and seedling vigor index. The total unsaturated fatty acids ranged from 85.02% to 87.31%. Meanwhile, total saturated fatty acids ranged from 13.41% to 15.46%. High estimates of (PCV) and (GCV) were exhibited for number of branches pl-1, number of capsules pl-1 and seed yield pl-1. The high heritability was exhibited for number of capsules pl-1, weight of 1000 seeds, seed vigor index, accelerated ageing germination, electrical conductivity µScm-1g-1, Seedling fresh weight and Seedling dry weight, so, selection could be effective for these characters. Heritability h2b was 70% at crude oil, 75% in crude protein and 87.51 % in total carbohydrate so; selection would be relatively effective for these traits. Randomly amplified polymorphic DNA (RAPD) analysis used to test the genetic diversity which generated a total of 52 DNA fragments, out of them 43 bands (82.7%) were polymorphic and high level of polymorphism was detected among sesame genotypes. RAPD-PCR was found to be useful as genotypes unique markers. The highest similarity was 88% between line108-6 and line111-4, While, line111-6 was genetically distant from line117-1 (similarity index 56%).References
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