CLONING OF WUB3a GENE (DROUGHT STRESS TOLERANT GENE) ISOLATED FROM Triticum aestivum
Abstract
The (ubiquitin-conjugating enzymes) E2s plays important role in response to various stresses in the plant, found in eukaryotes. The wub3a drought-tolerant gene coding to the E2 ubiquitin enzyme was isolated from the cDNA of double haploid 4 (DH4) genotype (triticum aestivum) under drought stress 30% PE and sequenced with 234bp complete open reading frame. The gene was deposited at GenBank under accession no. MW344069 and cloned in pBi121 expression vector and transformed in E. coli DH5α for preparing to transformation in the plant. The results illustrated that there is a different nucleotide sequence in the wub3a gene and the other sequence in GenBank while the translated protein was the same sequence. The gene requires more studies in the future.
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