IDENTIFICATION OF FRAGRANCE GENE fgr IN THE EGYPTIAN JASMINE RICE (EJR) AND ITS CROSSES WITH THREE CHINESE CYTOPLASMIC MALE STERILE LINES

Authors

  • AMIRA EL-KEREDY Genetics Department, Faculty of Agriculture, Tanta University
  • I., S. EL-DEGWY Crops Department, Faculty of Agriculture, Kafr El Sheikh University

Abstract

Aroma Egyptian Jasmine rice (EJR) is one of the most important types, It is one of the high quality rice varieties all over the world, have the fragrance gene fgr. Three cytoplasmic male sterile lines, and EJR were hybridized. The three cytoplasmic male sterility Chinese lines were: Hexi41B, Yimi15B and D297 and their crosses were Hexi41B x E.Jasmine (HJR), Yimi15B x E. Jasmine (YJR) and D297 x E. Jasmine (DJR). The results showed that the male sterile lines outperform the Egyptian Jasmine rice in most of morphological trail such as; 1000 grain weight, plant height, number of panicles plant, and grain yield for plant traits. For identification of fgr gene primers were used in PCR reaction, the fgr gene was identified in the Egyptian Jasmine rice (EJR) and its hybrid lines. The gene was also digested using three different restriction enzymes (RE) such as; EcoR1, BamH1 and Hind ІІІ. By estimating the total amount of protein in the grains, the result noticed that it reached 6.91 in the Egyptian Jasmine rice (EJR) and about 6.91(grain with cover), while it reached the highest amount in DJR hybrid as crosse between the Egyptian Jasmin rice (EJR) and male sterility lines leads to fgr gene transfer enhancement and increase of protein content in rice grains which reached up to 8.3 in D297 x Egyptian Jasmine rice hybrid (EJR). The main target of this study was to determine the fragrance gene (fgr) from EJR in cytoplasmic male strile (CMS) rice after crosses between them and the EJR, that resulting in changes of charachterstics after transferring the gene to these CMS lines.

References

Ahuja K. P., Jain U. and Jain .R. K., (2012). Fragrance analysis using molecular and biochemical methods in recombinant inbred lines of rice. African Journal of Biotechnology, 11 (91): 15784-15789.

Aliyu R. E., Wasiu A. A., Dangora D. B., Ademola J. O. and Adamu A. K., (2013). Comparative study of genomic DNA extraction protocols in rice species. International Journal of Advancements in Research & Technology, Vol.2, (2):ISSN: 2278-7763.

Bhat A. J., Ali. S., Salgotra R. K., Mir Z. A., Dutta S., JadonV., Tyagl A., Mushtaq M., Jain N., Singh P. K., Singh G. P. and Prabhu K. V., (2016). Genomic Selection in the Era of Next Generation Sequencing for Complex Traits in Plant Breeding. Frontiers in Genetics, Vol. 7: 221.

Bhattacharjee P., Singhal R. S. and Kulkarni P. K., (2002). Basmati Rice: A Review, Int. J. Food Sci., Technol., 37: 1.

Bourgis F., Guyot R.,Gherbi H., Talliez E., Amabile I., Salse J., Lorieux M., Delserry M. and Ghesquiere A., (2008). Characterization of the major fragance gene from an aromatic japonica rice and analysis of its diversity in Asian cultivated rice. Theor. Appl. Genet., 117: 353-368.

Bradbury L. M., Fitzgerald T. L., Henry R. J., Jin Q. and Waters D. L. E., (2005). The gene for fragrance in rice. Plant Biotechnology Journal, 3: 363-370.

Chen S., Wu. J., Yang Y., Shi W. and Xu M., (2006). The fgr gene responsible for rice fragrance was restricted within 69kb. Plant Sci., 171(4):505-14.

Choi J. Y., Groen S. C., Zaaijer S. and Purugganan M. D., (2018). Nanopore sequence-based genome assembly of the basmati rice. bio-Rxiv, 396515. doi: 10.1101/396515.

Chu S. C., Lee H. H., Ryu S. N. and Koh H. J., (2004). Grain characteristics and inheritance of green-kerneled rice (Oryza sativa L.). Korean J. Breed, 36(4): 222-228.

Civáň P., Craig H., Cox C. J. and Brown T. A., (2015). Three geographically separate domestications of Asian rice. Nat. Plants 1: 15164-15164. doi: 10.1038/nplants.2015.164.

Civáň P., Ali S., Batista-Navarro R., Drosou K., Ihejieto C., Chakraborty D., (2019). Origin of the aromatic group of cultivated rice (Oryza sativa L.) traced to the Indian Sub-continent. Genome Biol. Evol., 11 (3): 832-843. doi:10.1093/gbe/ evz039.

El-Keredy M. S., El-Weheishy M. M., El-Degwy I. S., Tan X. L. and Lijuan C., (2008). Combining ability of some hybrid rice varieties with Chinese bred cytoplasmic male sterile lines. Journal of Agricultural Research Kafr Elsheikh University Vol.34 No (1): 11-22.

El-Keredy M. S., Abdel Hafez A. G., El-Weihishy M. M. and El-Degwy I. S., (2003). Potential of hybrid rice production under Egyptian conditions, Egypt. J. Plant Breeding, 7(1): 471-486.

Garris J. A., Tai H., Coburn T., J., Kresovich S. and McCouch S., (2005). Genetic and structure and diversity in Oryza sativa L. Genetics society of America, 169: 1631-1638.

Hashemi F. S. G., Ismail M. R., Rafii M. Y., Aslani F., Miah G. and Muharam F. M., (2018). Critical multifunctional role of the betaine aldehyde dehydrogenase gene in plants.

Jewel Z. A., Patwary A. K., Maniruz-zaman S., Barua R. and Begum S. A., (2011). Physico-chemical and genetic analysis of aromatic rice (Oryza sativa L.) germplasm. The Agriculurists 9 (1& 2): 82-88

Jimenez, F. R., Maughan. P. J., Alvarez.,A., Kietlinski K. D., Smith. S. M., Pratt D. B., et al (2013). Assessment of genetic diversity in Peruvian Amaranth (Amaranthus caudatus and A. hybridus) germplasm using single nucleotide polymorphism markers. Crop Sci., 53: 532-541. doi: 10.2135/crop sci.2012. 07.0413.

Jin Q. S., Waters D., Cordeiro G. M., Henry R. J. and Reinke R. F., (2003). A single nucleotide polymorphism (SNP) marker linked to the fragrance gene in rice (Oryza sativa L.) plant Sci., 165: 359-364.

Juliano B. O. and Duff B., (1991). Rice grain quality as an emerging priaity in national rice. Rice Grain Quality Research, :55-64.

Kishor D. S. J., Chin J. H. and Jong K. H. (2020). Evaluation of whole-genome sequence, genetic diversity, and agronomic traits of Basmati rice (Oryza sativa L.). Frontiers in Genetics, 11: Article 86.

Kottearachehi N., S., Priyangani E. G. D. and Attanayaka D. P. S. T., (2010). Identification of fragrent gene, fgr, in traditional rice varieties of Srilanka. J. Natn. Sci. Foundation Srilanka, 38 (2): 139-143.

Liu W., Ghouri F., Yu H., Li X., Yu S., Shahid M. Q., (2017). Genome wide resequencing of newly developed Rice Lines from common wild rice (Oryza rufipogon Griff.) for the identification of NBS-LRR genes. PloS One 12 (7), e0180662. doi: 10.1371/Journal.Pone.0180662.

Matsuoka Y., Vigouroux Y., Goodman M. M, Sanchez G. J.. and Buckler E., (2002). A single domestication for maize shown by multilocus microsatellite genotyping. Proc. Natl. Acad. Sci., USA 99: 6080-6084.

Randhir S. and Pradhan K., (1981). Forage evaluation. First Published, Printox, New Dslhi, Dhawan Printing works.

Sakthivel K., Rani N. Shobha, Pandey M. K., Sivaranjani A. K. P., Neeraja C. N. and Balachandran S. M., (2009). Development of a simple functional marker for fragrance in rice and its validationin IndianBasmati and non-Basmati fragrant rice varieties. Mol. Breed., 24 (2): 185-190. doi: 10.1007/s11032-009-9283

Salgotra R. K, Millwood R. J., Agarwal S. and Stewart Jr.C. N., (2011). High-throughput functional marker assay for detection of Xa/xa and fgr genes in rice (Oryza sativa L.). Electrophoresis, 32: 2216-2222.

Sekhar B. P. S and Reddy G. M., (1982). Amino acid profiles in some scented rice varieties. Theoretical and Applied Gentics, 62: 35-37.

Serba D., Muleta K., Amand P., Bernardo A., Perumal R. and Bashir E., (2019). Genetic diversity, population structure, and linkage disequilibrium of pearl millet. Plant Genome. 12, 180091. doi: 10.3835/plantgenome2018.11.0091

Shehata S. M, Ammar M. H., Abdelkalik A. F. and Zayed B. A., (2009). Morphological, molecular and biochemical evaluation of Egyptian Jasmine rice variety and its M 5 derived mutations. Africa Journal of Biotechnology, 8 (22): 6110-6116.

Shobha Rani N., Pandey M. K. and Prasad I., (2006). Historical significance, grain quality features and precision breedind for improvement of export quality basmati varieties in India. J. crop Sci., :29-41

Sood B. C. and Siddiq E. A., (1978). A rapid technique for scent determination in rice. Indian J. Genet. Plant Breed., 38: 268-271.

Sun S. X., Gao F.Y., Lu X. J., Wu X. J., Wang X. D. and Ren G. J., (2008). Genetic analysis and gene fine mapping of aroma in rice (Oryza sativa.L. Cyperales, poaceae). Genetics and Molecular Biology 31 2: 532-538.

Wang Y. and Li J., (2005). The plant architecture of rice (Oryza sativa). Plant Mol. Biol., 59 (1): 75-84. doi: 10.1007/s11103-004-4038-x.

Widjaja R., Craske D. J. and Wootton M., (1996). Comparative studies on volatile components on-fragrant and fragrant Rices. J.S.c.i. Food Agric., 70: 151-161.

Yoshihashin T., (2002). Quantitative analysis on 2-Acetyl-1-pyrroline of an aromatic rice by stable isotope dilution method and models studies on its formation during cooking. Food Chemistry and Toxicology, 67: 619-622.

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2021-10-11

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