TRANSFER OF GLUCANASE GENE TO RESIST LATE BLIGHT DISEASE IN POTATO (Solanum tuberosum L.)

Authors

  • HEBA S. SHEBL Agricultural Genetic Engineering Research Institute, AGERI, ARC, Giza, Egypt
  • E. A. METRY Agricultural Genetic Engineering Research Institute, AGERI, ARC, Giza, Egypt
  • M. A. RASHED Genetics Department, Faculty of Agriculture, Ain Shams University, Shoubra Elkheima, Egypt
  • M. A. ABD EL-HAFIEZ Genetics Department, Faculty of Agriculture, Ain Shams University, Shoubra Elkheima, Egypt
  • I. M. ISMAIL Agricultural Genetic Engineering Research Institute, AGERI, ARC, Giza, Egypt

Abstract

Potato late blight is a pandemic disease caused by the highly virulent (Phytophthora infestans) fungus. The regeneration capacity was done among three tested potato cultivars (Spunta, Diamont and Desiree). Two different types of explants (inter-node and leaf) were cultured for calli induction and plant regeneration .The highest value of leaf explants was for Desiree cultivar (80%), which induced from developed callus in the 2,4-D medium. The best value of maximum shoot regeneration was also for Desiree cultivar in the BA, IAA and GA3 media from leaf which proved to be more effective. Then cloning of glucanase gene in pRI plasmid which carrying kanamycin resistance (nptII) gene was perfomed and followed by transformation in Agrobacterium tumefaciens LBA4404 strain which used for plant transfection. Nucleotide and amino acid sequences of transformed Agrobacterium were analyzed. The putative transgenic plantlets genomes and cDNA of the isolated RNA for glucanase gene.

References

Beaujean, A., R. Sangwan, A. Lecardonnel and B. Sangwan- Norreel (1998). Agrobacteriummediated transformation of three economically important potato cultivars using sliced internodal explants: an efficient protocol of transformation. Journal of Experimental Botany, 49: 1589- 1595.

Bowles, D. J. (1990). Defense-related proteins in higher plants. Annual Review of Biochemistry, 59: 873- 907.

Broglie, K. E., J. J. Gaynor and R. M. Broglie (1986). Ethylene-regulated gene expression: molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris. Proceedings of the National Academy of Sciences, 83: 6820-6824.

Cornelissen, B. J. and L. S. Melchers (1993). Strategies for control of fungal diseases with transgenic plants. Plant Physiology, 101: 709.

Fanta, N., X. Ortega and L. M. Perez (2003). The development of Alternaria alternata is prevented by chitinases and ß-1, 3-glucanases from Citrus limon seedlings. Biological Research, 36: 411-420.

Hoque, M. (2010). In vitro tuberization in potato (Solanum tuberosum L.). Plant Omics, 3: 7.

Hussain, J., C. Millman, J. Scott, P. Hannon and H. Matlay (2007). Ethnic minority graduate entrepreneurs in the UK characteristics, motivation and access to finance. Industry and Higher Education, 21: 455-463.

Lozovaya, V. V., A. Waranyuwat and J. M. Widholm (1998). β-l, 3- glucanase and resistance to Aspergillus flavus infection in maize. Crop Science, 38: 1255- 1260.

Matzke, M. and A. Matzke (1998). Epigenetic silencing of plant transgenes as a consequence of diverse cellular defence responses. Cellular and Molecular Life Sciences CMLS, 54: 94-103.

Murashige, T. and F. Skoog (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15: 473-497.

Nie, X. and R. Singh (2001). A novel usage of random primers for multiplex RT-PCR detection of virus and viroid in aphids, leaves, and tubers. Journal of Virological Methods, 91: 37-49.

Schickler, H. and I. Chet (1997). Heterologous chitinase gene expression to improve plant defense against phytopathogenic fungi. Journal of Industrial Microbiology and Biotechnology, 19: 196-201.

Solomon‐Blackburn, R. M. and H. Barker (2001). Breeding virus resistant potatoes (Solanum tuberosum): a review of traditional and molecular approaches. Heredity, 86: 17-35.

Spychalla, J. P. and M. W. Bevan (1993). Agrobacterium-mediated transformation of potato stem and tuber tissue, regeneration and PCR screening for transformation. Plant Tissue Culture Manual. Springer, p: 379-396.

Sreeramanan, S., M. Maziah, N. Rosli, M. Sariah and R. Xavier (2006). Particle bombardment-mediated co-transformation of chitinase and β-1, 3 glucanase genes in banana. Biotechnology, 5: 203-216.

Srivastava, A., L. Diengdoh, R. Rai and T. Bag (2012). Tissue culturetechnology harnessed for potato seed production. Keanean J. Sci., 1: 80-86.

Tam, D. M. and N. T. Lang (2003). In vitro selection for salt tolerance in rice. OmonRice, 11: 68-73.

Visser, R. G. (1991). Regeneration and transformation of potato by Agrobacterium tumefaciens. Plant Tissue Culture Manual. Springer, p: 301-309.

Yamamoto, T., H. Iketani, H. Ieki, Y. Nishizawa, K. Notsuka, T. Hibi, T. Hayashi and N. Matsuta (2000). Transgenic grapevine plants expressing a rice chitinase with enhanced resistance to fungal pathogens. Plant Cell Reports, 19: 639- 646.

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Published

2018-01-12

Issue

Vol. 46 No. 2 (2017)

Section

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