Solanaceae family is considered one of the major plant families providing food. Studies based on DNA and RNA molecular markers are more precise, reliable and powerful to assess the relationships between species also between genera in the same family. ITS sequences have been broadly used in the inference of phylogenetic hypotheses and in molecular evolution studies of plants, because it is variable, represent point mutations and insertions/deletions (indels). Specific ITS fragments were produced using the universal primer through seven sample of Solanaceae. Purified fragments were sequenced and each sequence was aligned individually at BLAST to confirm each species and to determine its relation to other sequence. Multiple Sequence alignment was done using Clustal Omega program for the seven sequences, and phylogenetic relationship showed that Potato was closely related to Tomato as they were grouped in one main cluster. Whereas Eggplant was related to both type of pepper (Chili and Bell) and was grouped in another main cluster. The Ground Cherry was separated alone in the third main cluster. Finally, ten related sequences picked from each alignment were deduced from BLAST to make the multiple alignments for the seven studied samples with other species of Solanaceae family.

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Barchi, L., S. Lanteri, E. Portis, A. Acquadro, G. Vale, L. Toppino and G. L. Rotino (2011). Identification of SNP and SSR markers in eggplant using RAD tag sequencing. BMC Genomics, 12: 304.

Barone, A., A. Sebastiano, D. Carputo, F. D. Rocca and L. Frusciante (2001). Molecular marker-assisted introgression of the wild Solanum commersonii genome into the cultivated S. tuberosum gene pool. Theor. Appl. Genet., 102: 900-907.

Bebeli, P. J. and A. Mazzucato (2008). The Solanaceae -a review of recent research on genetic resources and advances in the breeding of tomato, pepper and eggplant. The European Journal of Plant Science and Biotechnology, 2: 3-30.

Finger, F. L., S. D. Lannes, A. R. Schuelter, J. Doege, A. P. Comerlato, L. S. A. Gonçalves, F. R. A. Ferreira, L. R. Clovis and C. A. Scapim (2010). Genetic diversity of Capsicum chinensis (Solanaceae) accessions based on molecular markers and morphological and agronomic traits. Genetics and Molecular Research, 9: 1852- 1864.

Giudicelli, G. C., G. Mäder, G. A. Silva- Arias, P. M. Zamberlan, S. L. Bonatto and L. B. Freitas (2016). Secondary structure of nrDNA internal transcribed spacers as a useful tool to align highly divergent species in phylogenetic studies. Genetics and Molecular Biology, Ahead of Print Epub. Feb. 13, 2017.

Gramazio, P., J. Blanca, P. Ziarsolo, F. J. Herraiz, M. Plazas, J. Prohens and S. Vilanova (2016). Transcriptome analysis and molecular marker discovery in Solanum incanum and S. aethiopicum, two close relatives of the common eggplant (Solanum melongena) with interest for breeding. BMC Genomics, 17: 300-317.

Hoque, M. E., H. Huq and N. J. Moon (2013). Molecular diversity analysis in potato (Solanum tuberosum L.) through RAPD markers. SAARC J. Agri., 11: 95-102.

Poczai, P., I. Cernák, A. M. Gorji, S. Nagy, J. Taller and Z. Polgár (2010). Development of intron targeting (IT) markers for potato and cross-species amplification IN Solanum nigrum (Solanaceae). American Journal of Botany, 97: 142- 145.

Samsuddin, M., N. Mat, N. Z. Mazlan, S. N. I. Omer, S. Tajuddin, N. Ngah and A. M. Ali (2012). Internal transcribed spacer (ITS) as molecular marker for identification of Dioscorea hispida Dennst. (Dioscoreaceae). J. Agrobiotech., 3: 47-55.

Samuels, J. (2015). Biodiversity of food species of the Solanaceae family: a preliminary taxonomic inventory of subfamily solanoideae. Resources, 4: 277-322.

Sharma, S., S. Rustgi, H. S. Balyan and P. K. Gupta (2002). Intraspecific sequence variation in the internal transcribed spacer (ITS) region of ribosomal DNA in common wheat and wild barley. Barley Genetics Newsletter, 32: 38-45.

Shirasawa, K. and H. Hirakawa (2013). DNA marker applications to molecular genetics and genomics in tomato. Breeding Science, 63: 21- 30.

Wei, J., X. Hu, J. Yang and W. Yang (2012). Identification of singlecopy orthologous genes between Physalis and Solanum lycopersicum and analysis of genetic diversity in Physalis using molecular markers. PLoS ONE, 7: 50164-50173.

Yadav, R., M. Rathi, A. Pednekar and Y. Rewachandani (2016). A Detailed review on Solanaceae family. European Journal of Pharmaceutical and Medical Research, 3: 369-378.


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