DETECTION OF SOMACLONAL VARIATIONS IN TOMATO USING RAPD MARKERS

A. A. ALI, M. E. EL-DENARY, A. EL-GENDY, OLA A. GALAL, M. E. AHMAD, TAHANY R. EL-SAYED

Abstract


Eleven random primers were used to study somaclonal variation among sixteen somaclones derived from four parental genotypes (Super Strain B, Castle Rock, Advanttage II and Edkawy). Out of eleven random primers used, only six primers (OPA01, OPA02, OPA03, OPA04, OPA05 and OPB11) successfully produced scoreable RAPD bands for all the tested genotypes. DNA amplification with the six different primers generated 285 bands 130 of them were polymorphic and 155 monomorphic bands in all the genotypes studied. Among the primers used, OPA05 produced the highest number of bands (16 bands) while primers OPA04 produced the lowest number (12 bands). RAPD patterns generated by these primers achieved high polymorphic percentage, indicating high level of genetic variations among somaclones and their parental genotypes. Super Strain B cultivar showed the highest number of polymorphic percentage 54.8%, while Advanttage II and Edkawy cultivars showed the lowest percentage of somaclonal variations 42.3% and 42.2%, respectively. The relatively high frequency of variations detected here might bias stable plant propagation. However it could be regarded as a novel source of tomato improvement.

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