EXPERIMENT ON THE GENETIC TOXICITY OF TARTRAZINE YELLOW AND BEHAVIORAL EFFECTS ON Drosophila melanogaster

AMIRA EL-KEREDY

Abstract


The aimed of this study is to know harmful effects of tartrazine (yellow 5- E102) on chromosomes and behavior in a natural Egyptian population of Drosophila melanogaster from Tanta, Egypt. Five concentrations of tartrazine (1, 2.5, 5, 7.5 and 10%) previously were used for five generations. Each generation was allowed to reproduce for 12 days under tartrazine exposure. Additionally, tartrazine effects (toxicity) on a long term of the male and female lethal flies were detected, and it had an impact on the ratio between male and female (sexual ratio). Chi-square statistic at 0.05 level of significance showed that there are significant difference on the sexual ratio between males and females (χ2 = 6.0) at the 5% Tart., concentration in the fifth generation. Inversions 3L(P) and 3R(C) were detected only after treatment with tartrazine concentrations in fifth generations at the Cytological part in this study. The dose-effect- behavioral functions (learning) for tartrazine concentrations determined that high dose reduced insects ability to learn which affects in his behavior. Results of the study showed that tartrazine concentrations increased the rate of toxicity, mutations, genotoxicity, disruption of sex ratio and the ability to learn was lost.

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Elhakim, O. M., F. Heraud, N. Bemrah, F. Gauchard, T. Lorino, C. Lambre, M. J. Fremy, and M. J. Poul (2007). New considerations regarding the risk assessment on Tartrazine an update toxicological assessment, intolerance reactions and maximum theoretical daily intake in France. Regulatory Toxicology and Pharmacology 47: 308-316.

El-Keredy, A. (2014). Genetic and behavioral influences of quinine and monosodium glutamate on Drosophila melanogaster. Egypt. J. Genet. Cytol., 43: 377-391.

El-Keredy, A., M. Schleyer, C. Konig, A. Ekim and B. Gerber (2012). Behavioural analyses of quinine processing in choice, feeding and learning of larval Drosophila. PLOS ONE 7:e40525.

Fiala, A., T. Spall, S. Diegelmann, B. Eisermann, S. Sachse, M. M. J. Devand, E. Buchner and G. C. Galizia (2002). Genetically expressed cameleon in Drosophila melanogaster is used to visualize olfactory information in projection neurons. Current Biology, 12: 1877-1884

Gerber, B. and R. F. Stocker (2007). The Drosophila Larva as a Model for Studying Chemosensation and Chemosensory Learning. A Review Chem. Senses, 32: 65-89.

Gerber, G. J. and D. O'Shaughnessy (1986). Comparison of the behavioral effects of neurotoxic and systemically toxic agents: how discriminatory are behavioral tests of neurotoxicity? Neurobehavioral Toxicology and Teratology, 8: 703- 710.

Ishidate, M., T. Sofuni, K. Yoshikawa, M. Hayashi, T. Nohmi, M. Sawada and A. Matsuka (1984). Primary mutagenicity screening of food additives currently used in Japan. Food Chem. Toxic., 22: 623-636.

Khurana, S., B. M. AbuBaker and O. Siddiq (2009). Odour avoidance learning in the larva of Drosophila melanogaster. J. Biosci., 34: 621- 631.

Kulig, B., E. Alleva, G. Bignami, J. Cohn, C. D. Slechta,.V Landa, O. J. Donoghue and D. Peakall (1996). Animal Behavioral Methods in Neurotoxicity Assessment: SGOMSEC Joint Report. Environmental Health Perspectives, 104: 193-204.

Liang, L. and L. Liqun (2010). The olfactory circuit of the fruit fly Drosophila melanogaster. Sci. China Life Sci., 53: 472-484.

Lindsley, D. L. and E. H. Grell (1968). Genetic variations of Drosophila melanogaster. Carnegie Institution of Washington Publ.627, Washington, DC.

Niewalda, T., N. Singhal, A. Fiala, T. Saumweber, S. Wegener and B. Gerber (2008). Salt processing in larval Drosophila: choice, feeding, and learning shift from appetitive to aversive in a concentrationdependent way. Chemical Senses, 33: 685-692.

Russell, C., J. Wessnitzer, J. M. Young, J. D. Armstrong and B. Webb (2011). Dietary salt levels affect salt preference and learning in larval Drosophila. PLOS ONE 6:e20100.

Sarikaya, R. and S. Cakn (2005). Genotoxicity testing of four food preservatives and their combinations in the Drosophila wing spot test. Environmental Toxicology and Pharmacology, 20: 424-430.

Sasaki, Y. F., S. Kawaguchi, A. Kamaya, M. Ohshita, K. Kabasawa, K. Iwama, K. Taniguchi and S. Tsuda (2002). The comet assay with 8 mouse organs: results with 39 currently used food additives. Mutat. Res., 519: 103-119.

Schleyer, M., S. Diegelmann, B. Michels, T. Saumweber and B. Gerber (2013). Decision-making in larval Drosophila. In: Menzel R., Benjamin P., editors. Invertebrate learning and memory. München: Elsevier. p. 41-55.

Schleyer, M., D. Miura, T. Tanimura and B. Gerber (2015). Learning the specific quality of taste reinforcement in larval Drosophila. Neuroscience. 4:e04711. DOI: 10.7554/eLife.04711

Silva, B., M. C. Fernandez, B. M. Ugalde, I. E. Tognarelli, C. Angel and M. J. Campusano (2015). Muscarinic ACh Receptors Contribute to Aversive Olfactory Learning in Drosophila. Neural Plasticity, Article ID 658918, 10.

Scherer S., F. R. Stocker and B. Gerber (2003). Olfactory learning in individually assayed Drosophila larvae. Learning and Memory. Learn. Mem., 10: 217-225.

Scott, K., R. Brady, A. J. Cravchik, P. Morozov, A. Rzhetsky, C. Zuker, and R. Axel (2001). A Chemosensory Gene Family Encoding Candidate Gustatory and Olfactory Receptors in Drosophila. Cell, 104: 661-673.

Tantiado, G. R. (2012). Comparative mutagenic effects of laboratory dyes on Drosophila melanogaster. International Journal of Bio-Science and Bio-Technology Vol. 4, No. 4: 55-62

Tennant, R. W. (2009). Mutagens and Carcinogens. Retrieved from http://www.accessscience.com/pop up.aspx?id=441100&name=print

Tennant, R. W. (2014). Mutagens and Carcinogens. Biology & Biomedicine Genetics. DOI:http://dx.doi. org/10.1036/1097-8542.441100

Yan, Q. D. (2008). Experiment on the acute toxicity and genetic toxicity of tartrazine on Paramisgurnus dabraynus. Journal of Anhui Agricultural Sciences, 15

Yanzhi, W., G. Meijiang, L. Yiqiu, Li Jingtian, He Linxin, D. Chunyan (2012). Tartrazine on SOD Activity and Genetic Effects in Drosophila melanogaster. Journal of the Graduates Sun Yat-Sen University, (Natural Sciences、Medicine. 03.


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