MUSCLE PROTEIN AND LIVER ESTERASES BANDING PAT-TERNS AS BIOCHEMICAL MARKERS TO DETERMINE GENETIC DIVERSITY IN EGYPTIAN POPULATIONS OF Tilapia SPECIES

Authors

  • G. EL-FADLY Department of Genetics, Faculty of Agriculture, Kafr El-Sheikh University, 33516, Egypt
  • M. REHAN Department of Genetics, Faculty of Agriculture, Kafr El-Sheikh University, 33516, Egypt
  • I. KHATAB Department of Genetics, Faculty of Agriculture, Kafr El-Sheikh University, 33516, Egypt
  • A. KALBOUSH Department of Genetics, Faculty of Agriculture, Kafr El-Sheikh University, 33516, Egypt

Abstract

Genetic diversity across Tilapia species is important key fordevelopment aquaculture strains, protection of endangered populations and biogeographical inferences. Total soluble protein and esterases isozymes were extracted from flesh (muscles) and liver of all individuals from the three populations (Ryad, Bahr El-Baqar and Motobs) from each species under study to estimate the genetic diversity. With comparing the obtained bands from the three Oreochromis niloticus, it was found that 13 bands out of the 20 were common bands among the three populations. The three T. zilli populations exhibited 8 bands out of the 21 were common bands among those three populations. The three O. aurea populations exhibited 10 bands were common between three populations, while the rest of bands appeared in some of population and disappeared in the other. Ryad and Motobs individuals of O. niloticus showed four isozymes. Meanwhile, Bahr El-Baqar exhibited only three. Ryad and Bahr El-Baqar individuals of T. zilli showed three isozymes. Meanwhile, Motobs exhibited four ones. Maximum two isozymes were detected in population of O. aurea. Band 1 was dark in Ryad individuals and ranged from very faint, faint and dark in Bahr El-Baqar and Motobs. The phylogenetic relationship within the studied populations of the three locations concerning O. niloticus, T. zilli and O. aurea was conducted and different variations were detected. Population from Ryad was highly differentiated than other populations.

References

Badawy, E. (1998). Oreochromis niloticus and offsprings of Red Tilapia in Egypt: genetic prospective. Egypt. Journal of Aquatic Biology and Fisheries, 3: 123-131.

Bakhoum, Sh., M. Ahmed and E. Ragheb (2009). Genetic evidence for natural hybridization between Nile Tilapia (Oreochromis niloticus; Linnaeus, 1757) and Blue Tilapia (Oreochromis aureus; Steindachner, 1864) in Lake Edku, Egypt. Global Veterinaria, 3: 91-97.

Barua, S., M. Alam and V. Simonsen (2004). Genetic variation in four hatchery populations of Thai pangas, Pangasius hypothalamus of Mymensingh region in Bangladesh using allozyme marker. Pakistan Journal of Biological Sciences, 7: 144-149.

Bezrukov, V. (1987). Esterase isozymes and their variation in grass Carp tsitologiya. Genetika, 21: 293-297.

Debnath, J. (1978). Electrophoretic and Biochemical studies of proteins and isozymes of non-specific esterase, Lactate and Malate dehydrogenases in the three species of freshwater fishes of Bosnia and Hercegovina. Univ. Medical Centre, Sarajevo. (PhD Thesis).

El-Fadly, G., S. Sidaros and A. Dif (1990). Effect of bovistin on gene expression and yield components of faba bean (Vicia faba) infected with broad bean strain virus. Proc. 3rd Conf. Agric. Dev. Res., Fac. Agric., Ain Shams Univ., Cairo, Egypt.

El-Ghobashy, H., M. A. Rashed, S. Mohamed and A. Ramadan (2005). protein and isozymes electrophoresis for identifying some carp species. The Egyptian Society of Experimental Biology (Zoology), 1: 65-72.

El-Tawil, M. (1984). Distribution of lactate dehydrogenase isozyme patterns of gobioid fishes. Marine Biology Research Center Tripoli, Libya, 5: 5-35.

Eshak, M., N. Ebeed, H. Booles and K. Fahmy (2010). Genetic assessment of salinity tolerance in Red Tilapia. New York Science Journal, 3: 132-141.

Hammer, Ø, D. Harper and P. Ryan (2001). PAST: Palenotological statistics software package for education and data analysis. Palentologia Electronica, 4: 1-9. http://palaeo-electronica.org/2001_1/past/issue1_01.htm.

Jean, C., S. Lee, C. Hui and C. Chen (1995). Tissue-specific isozymes in fishes of the subfamily Sparinae (Perciformes: Sparidae) from the Coastal Waters of Taiwan. Zoological Studies, 34: 164-169.

Kijima, A. and Y. Fujio (1990). Genetics analysis of population structure in marine Teleost around Japan. Progress in Clinical and Biological Research, 344: 177-206.

Ladewig, P. and L. Schwantes (1984). Loci that encode the lactate dehydrogenase in 23 species of fish belonging to the orders Cypriniformes, Siluriformes and Perciformes: Adaptative features. Comparative Biochemistry and Physiology, 77: 867-876.

Laemmli, U. (1970). Cleavage of structural protein during assembly of head bacteriophage T4. Nature, 227: 680-685

Mugiyanto, S., D. Arfiati, A. Prajitno and Marsoedi (2013). Esterase isozyme banding pattern of Anguilla marmorata from four rivers of kaur Distict, Benkgulu. International Journal of Research in Fisheries and Aquaculture, 3: 78-80.

Mustafa, M., A. Rashid, Z. Ferdous, M. Alam, R. Begum and S. Reza (2013). Tissue specific esterase isozyme variation in four Punctius species. Journal of Current Research in Science, 1: 254-259.

Rashed, M., E. Badawy, Y. Saad and A. Abd EL-Rasek (2007). Genetic signature of some Hemichromis bimaculatus fish populations based on muscle protein polymorphism. Egyptian Journal of Aquatic Biology and Fisheries, 11: 83-100.

Rashid, A. and D. Rahman (2013). Tissue specific esterase isozyme variation in Clarias batrachus and C. gariepinus. Journal of Entomology and Zoology Studies, 1: 1-7.

Rognon, X., M. Andriamanga, B. Mcandrew and R. Guyomard (1996). Allozyme variation in natural and cultured populations in two Tilapia species: Oreochromis niloticus and Tilapia zilli. Heredity, 76: 640-650.

Saad, Y., M. Hanafi, M. Essa, A. Guerges and F. Ali (2009). Genetic signature of some Egyptian Clarias gariepinus populations. Global Veterinaria, 3: 503-508.

Sahib, I. and K. Rao (1980). Toxicity of malathion to the freshwater fish Tilapia mossambica. Bulletin of Environmental Contamination and Toxicology, 24: 870-874.

Saxena, A. and N. Soranganba (2012). Esterases diversity in Indian and Exotic carps. Trends in Fisheries Research, 1: 24-28.

Scandalios, J. G. (1964). Tissue-specific isozyme variation in maize. J. of Heredity, 55: 281-285.

Shahjahan, R., A. Karim, R. Begum, M. Alam and A. Begum (2008). Tissue specific esterase isozyme banding pattern in Nile Tilapia (Oreochromis niloticus). Rajshahi University Zoological Society, 27: 1-5.

Shengming, H., Q. Changgeng and T. Thukui (1988). Comparative studies on the electrophoregram of esterase isozyme and lactate dehydrogenase of Carassius aukatus gibelio bloch and Carassius sp. Zoology Research, 9: 69-78.

Silva, C., R. Lima and A. Teixeira (2008). Isoenzyme electrophoretic patterns in discus fish from the Central Amazon. Genetics and Molecular Research, 7: 791-805.

Stegmann, H., W. Burgermeister, H. Franksen and E. Krojerrecklenfort (1985). Polyacrylamide gel electrophoresis manual, Chapter 3, page 6 and chapter 5, page 1.

Downloads

Published

2017-01-19

Issue

Section

Articles

Most read articles by the same author(s)